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Pcr : NEET MCQ Questions

Are you looking for NEET MCQ Questions on Pcr? If Yes then you are at right place. Here you will find Pcr NEET MCQ Questions and Answers, Trivia, Quiz, Interview MCQ & Pcr Current Affairs for Competitive Exams like Neet exams, around 30% to 40% of Neet questions are asked from given Pcr Mcqs. as you know Questions and Ansers on Pcr MCQS is very important for Neet Exam. In this Pcr NEET section for Neet competition we are covering NEET MCQ Questions on Pcr

Question 1 : The polymer chain reaction is used for_________.

A. Amplifying gene of interest

B. Constructing RAPD maps

C. Detecting the presence of the transgene in an organism

D. All of the above

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Question 2 : Which of the following is true for asymmetric PCR?

A. Used for generating double-stranded copies for DNA sequence

B. Used for generating single-stranded copies for DNA sequence

C. Both a and b

D. None of the above

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Question 3 : Which of the following is an application of polymer chain reaction?

A. Site-directed mutagenesis

B. Site-specific recombination

C. Site-specific translocation

D. All of the above

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Question 4 : Reverse transcription PCR uses _________.

A. RNA as a template to form DNA

B. mRNA as a template to form cDNA

C. DNA as a template to form ssDNA

D. All of the above

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Question 5 : At what temperature does annealing of DNA and primer take place?

A. 54°C

B. 96°C

C. 42°C

D. 74°C

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Question 6 : Primers used for the process of polymerase chain reaction are _________.

A. Single-stranded RNA oligonucleotide

B. Single-stranded DNA oligonucleotide

C. Double-stranded RNA oligonucleotide

D. Single-stranded DNA oligonucleotide

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Question 7 : How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?

A. 8

B. 4

C. 32

D. 16

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Question 8 : At what temperature does denaturation of DNA double helix take place?

A. 54°C

B. 74°C

C. 94°C

D. 60°C

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Question 9 : Polymerase used for PCR is extracted from _____________ .

A. Homo sapiens

B. Thermus aquaticus

C. Escherichia coli

D. Saccharomyces cerevisiae

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Question 10 : Which of the following statements are true regarding PCR?

A. Primer extension occurs at 72°C

B. Denaturation involves heating at 90 to 98°C

C. Annealing involves the binding of primer between 40 to 60C°C

D. All of the above

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Question 11 : Denaturation is the process of _________.

A. Heating between 72°C

B. Heating between 40 to 60°C

C. Heating between 90 to 98°C

D. None of the above

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Question 12 : What is the process of binding of primer to the denatured strand called?

A. Annealing

B. Renaturation

C. Denaturation

D. None of the above

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Question 13 : Which of the following is the first and the most important step in the polymerase chain reaction?

A. Annealing

B. Primer extension

C. Denaturation

D. None of the above

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Question 14 : Why are vent polymerase and Pfu more efficient than the Taq polymerase?

A. Because of proofreading activity

B. Because of more efficient polymerase activity

C. Both A and B

D. None of the above

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Question 15 : Which of the following is the basic requirement of PCR reaction?

A. Two oligonucleotide primers

B. DNA segment to be amplified

C. A heat-stable DNA polymerase

D. All of the above

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Question 16 : Which of the following is not a thermostable polymerase?

A. pfu polymerase

B. Taq polymerase

C. Vent polymerase

D. DNA polymerase III

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Question 17 : Thermus aquatics is the source of _________.

A. Vent polymerase

B. Primase enzyme

C. Taq polymerase

D. Both a and c

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Question 18 : Which of the following statements is accurate for the PCR – polymerase chain reaction?

A. Automated PCR machines are called thermal cyclers

B. A thermostable DNA polymerase is required

C. Millions to billions of desired DNA copies can be produced from microgram quantities of DNA

D. All of the above

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Question 19 : The polymerase chain reaction is_________.

A. It is a DNA sequencing technique.

B. It is a DNA degradation technique

C. It is a DNA amplification technique

D. All of the above

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Question 20 : The PCR technique was developed by_________.

A. Kohler

B. Altman

C. Milstein

D. Kary Mullis

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