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Question : For what purpose have stirred tank bioreactors been designed?
A. addition of preservatives to the product
B. purification of the product
C. ensuring anaerobic conditions in the culture vessel
D. availability of oxygen throughout the process
Question : When a plasmid is used as a vector, what is its most important feature?
A. Origin of replication (Ori)
B. presence of a selectable marker
C. presence of sites for restriction endonuclease
D. its size
Question : What enzyme joins the ends of two strands of nucleic acid?
A. polymerase
B. synthetase
C. helicase
D. ligase
Question : With what method became it possible to link the antibiotic resistance gene to the plasmid vector?
A. DNA ligase
B. Endonucleases
C. DNA polymerase
D. Exonucleases
Question : Why is chilled ethanol added during the process of isolation of DNA?
A. Remove proteins such as histones
B. Precipitate DNA
C. Break open the cell to release DNA
D. Facilitate action of restriction enzymes
Question : Who was the first person to develop the DNA fingerprinting technique?
A. Boysen and Jensen
B. Edwards and Steptoe
C. Schleiden and Schwann
D. Jeffreys, Wilson and Thien
Question : Paul Bery was the first to synthesize recombinant DNA molecules using DNA of?
A. TMV and Salmonella
B. Virus SB-40 and E.coli
C. Influenza virus and Diplococcus bacterium
D. TMV and Agrobacterium tumefaciens
Question : Animal cell culture technology is used today most extensively in the production of what?
A. edible proteins
B. insulin
C. interferons
D. vaccines
Question : What is a single strand of nucleic acid tagged with a radioactive molecule called?
A. Vector
B. Selectablemarker
C. Plasmid
D. Probe
Question : In the context of observing DNA separated by agarose gel electrophoresis, which of the given statements is correct?
A. DNA can be seen in visible light
B. DNA can be seen without staining in visible light
C. Ethidium bromide-stained DNA can be seen in visible light
D. Ethidium bromide-stained DNA can be seen under exposure to UV light
Question : Suppose an electrical current has moved DNA fragments through a gel. What does the band on this gel that is furthest from the top (that is, from where the DNA fragments were added) represent?
A. shortest fragments of DNA
B. longest fragments of DNA
C. restriction enzyme used to cut the DNA into fragments
D. ligase used to bind the DNA fragments together
Question : Which of the following pairs is incorrectly matched?
A. Plasmid – Small piece of extrachromosomal DNA in bacteria
B. Interferon – An enzyme that interferes with DNA replication
C. Cosmid – A vector for carrying large DNA fragments into host cells
D. Myeloma – Antibody – producing tumour cells
Question : Which of the following is associated with reporter genes?
A. include genes for drug resistance
B. include genes for bioluminescence
C. include genes for DNA origins
D. Both a and b
Question : What does genetic engineering aim to achieve?
A. destroying wild gene
B. preserving defective gene
C. curing human disease by introducing a new gene (Haemophilia)
D. all the above
Question : Which of the following statements is not true about restriction enzymes?
A. It recognises a palindromic nucleotide sequence
B. it is an endonuclease
C. It is isolated from viruses
D. It produces the same kind of sticky ends in different DNA molecules