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Biotechnology principles and processes : NEET MCQ Questions

Are you looking for NEET MCQ Questions on Biotechnology principles and processes? If Yes then you are at right place. Here you will find Biotechnology principles and processes NEET MCQ Questions and Answers, Trivia, Quiz, Interview MCQ & Biotechnology principles and processes Current Affairs for Competitive Exams like Neet exams, around 30% to 40% of Neet questions are asked from given Biotechnology principles and processes Mcqs. as you know Questions and Ansers on Biotechnology principles and processes MCQS is very important for Neet Exam. In this Biotechnology principles and processes NEET section for Neet competition we are covering NEET MCQ Questions on Biotechnology principles and processes

Contents

Question 1 : For what purpose have stirred tank bioreactors been designed?

A. addition of preservatives to the product

B. purification of the product

C. ensuring anaerobic conditions in the culture vessel

D. availability of oxygen throughout the process

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Question 2 : When a plasmid is used as a vector, what is its most important feature?

A. Origin of replication (Ori)

B. presence of a selectable marker

C. presence of sites for restriction endonuclease

D. its size

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Question 3 : What enzyme joins the ends of two strands of nucleic acid?

A. polymerase

B. synthetase

C. helicase

D. ligase

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Question 4 : With what method became it possible to link the antibiotic resistance gene to the plasmid vector?

A. DNA ligase

B. Endonucleases

C. DNA polymerase

D. Exonucleases

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Question 5 : Why is chilled ethanol added during the process of isolation of DNA?

A. Remove proteins such as histones

B. Precipitate DNA

C. Break open the cell to release DNA

D. Facilitate action of restriction enzymes

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Question 6 : Who was the first person to develop the DNA fingerprinting technique?

A. Boysen and Jensen

B. Edwards and Steptoe

C. Schleiden and Schwann

D. Jeffreys, Wilson and Thien

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Question 7 : Paul Bery was the first to synthesize recombinant DNA molecules using DNA of?

A. TMV and Salmonella

B. Virus SB-40 and E.coli

C. Influenza virus and Diplococcus bacterium

D. TMV and Agrobacterium tumefaciens

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Question 8 : Animal cell culture technology is used today most extensively in the production of what?

A. edible proteins

B. insulin

C. interferons

D. vaccines

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Question 9 : What is a single strand of nucleic acid tagged with a radioactive molecule called?

A. Vector

B. Selectablemarker

C. Plasmid

D. Probe

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Question 10 : In the context of observing DNA separated by agarose gel electrophoresis, which of the given statements is correct?

A. DNA can be seen in visible light

B. DNA can be seen without staining in visible light

C. Ethidium bromide-stained DNA can be seen in visible light

D. Ethidium bromide-stained DNA can be seen under exposure to UV light

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Question 11 : Suppose an electrical current has moved DNA fragments through a gel. What does the band on this gel that is furthest from the top (that is, from where the DNA fragments were added) represent?

A. shortest fragments of DNA

B. longest fragments of DNA

C. restriction enzyme used to cut the DNA into fragments

D. ligase used to bind the DNA fragments together

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Question 12 : Which of the following pairs is incorrectly matched?

A. Plasmid – Small piece of extrachromosomal DNA in bacteria

B. Interferon – An enzyme that interferes with DNA replication

C. Cosmid – A vector for carrying large DNA fragments into host cells

D. Myeloma – Antibody – producing tumour cells

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Question 13 : Which of the following is associated with reporter genes?

A. include genes for drug resistance

B. include genes for bioluminescence

C. include genes for DNA origins

D. Both a and b

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Question 14 : What does genetic engineering aim to achieve?

A. destroying wild gene

B. preserving defective gene

C. curing human disease by introducing a new gene (Haemophilia)

D. all the above

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Question 15 : Which of the following statements is not true about restriction enzymes?

A. It recognises a palindromic nucleotide sequence

B. it is an endonuclease

C. It is isolated from viruses

D. It produces the same kind of sticky ends in different DNA molecules

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Question 16 : What is an example of gene therapy?

A. the production of injectable hepatitis B vaccine

B. production of vaccines in food crops like potatoes which can be eaten

C. introduction of gene for adenosine deaminase in persons suffering from Severe Combined Immuno Deficiency (SCID)

D. production of test-tube babies by artificial insemination and implantation of fertilized eggs

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Question 17 : Antibiotics are used in genetic engineering

A. as selectable markers

B. to select healthy vectors

C. to keep the cultures free of infection

D. as sequences from where replication starts

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Question 18 : The most commonly used vectors for human genome sequencing are?

A. T-DNA

B. BAC and YAC

C. Expression Vectors

D. kT/A Cloning Vectors

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Question 19 : The PCR (Polymerase Chain Reaction) was popularized by which of the following?

A. Easy availability of DNA template

B. Availability of synthetic primers

C. Availability of cheap deoxyribonucleotides

D. Availability of ‘Thermostable’ DNA polymerase

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Question 20 : Which of the following statements about cloning vectors is false?

A. ‘Ori’ is a sequence responsible for controlling the copy number of the linked DNA

B. Selectable marker selectively permitting the growth of the non-transformants

C. To link the alien DNA, the vector needs to have a single recognition site for the commonly used restriction enzymes

D. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes

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Question 21 : Which enzyme catalyzes the removal of nucleotides from the ends of DNA?

A. endonuclease

B. exonuclease

C. DNA ligase

D. Hind II

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Question 22 : What is a genetic marker?

A. a place where a restriction enzyme cuts DNA

B. a chart that traces the family history of a genetic trait

C. nucleotide sequence near a particular gene

D. radioactive probe used to find a gene

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Question 23 : If one were to produce large amounts of a recombinant protein, which of the following would produce the best yield?

A. Laboratory flask of the largest capacity

B. A stirred-tank bioreactor without in-lets

C. A continuous culture system

D. Any of the above

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Question 24 : What does the enzyme, restriction endonucleases do?

A. make cuts at specific positions within the DNA molecule

B. recognize a specific nucleotide sequence for binding of DNA ligase

C. restrict the action of the enzyme DNA polymerase

D. remove nucleotides from the ends of the DNA molecule

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Question 25 : In the Southern blot technique, what is the first step?

A. Denaturation of DNA on the gel for hybridization with a specific probe

B. Production of a group of genetically identical cells

C. Digestion of DNA by a restriction enzyme

D. Denaturation of DNA from a nucleated cell such as the one from the scene of a crime

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Question 26 : What is the source of the Taq polymerase enzyme?

A. Thermus aquaticus

B. Thiobacillus ferroxidans

C. Bacillus subtilis

D. Pseudomonas putida

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Question 27 : What is the purpose of the polyethylene glycol method?

A. biodiesel production

B. seedless fruit production

C. energy production from sewage

D. gene transfer without a vector

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Question 28 : What do vectors include?

A. bacterial and plant plasmids

B. viruses

C. artificial chromosomes

D. All of the above

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Question 29 : What are the characteristics of plant clonal propagation in vitro?

A. PCR and RAPD

B. Northern blotting

C. Electrophoresis and HPLC

D. Microscopy

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Question 30 : What was the first clinical gene therapy used to treat?

A. chickenpox

B. diabetes mellitus

C. rheumatoid arthritis

D. adenosine deaminase deficiency

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Question 31 : When separated on an agarose gel, DNA fragments can be visualized after staining with?

A. Acetocarmine

B. Aniline blue

C. Ethidium bromide

D. Bromophenol blue

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Question 32 : Which of these is incorrectly matched?

A. Gene gun—biolistic gun

B. Plasmids—extrachromosomal DNA

C. DNA ligase—Biological scissors

D. Bacteriophages—viruses

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Question 33 : Which of the following is a true statement about DNA polymerase that is used in PCR?

A. It is used to ligate introduced DNA in the recipient cell

B. It serves as a selectable marker

C. It is isolated from a virus

D. It remains active at high temperature

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Question 34 : Which of the following is used in genetic engineering?

A. Plasmid

B. Plastid

C. Mitochondria

D. E.R.

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Question 35 : What is the PCR (Polymerase Chain Reaction) method used for?

A. amplification of DNA for forming billions of copies of itself

B. monoclonal antibody production

C. hybridoma production

D. All of the above

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Question 36 : What role does DNA ligase play in the construction of a recombinant DNA molecule?

A. formation of a phosphodiester bond between two DNA fragments

B. formation of hydrogen bonds between sticky ends of DNA fragments

C. ligation of all purine and pyrimidine bases

D. None of the above

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Question 37 : In which DNA sequence does the restriction enzyme EcoRI always cleave the nitrogen sequence?

A. AAGCTT

B. GGATCC

C. GAATTC

D. TGGCCA

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Question 38 : What does cloning means?

A. all of the cells are derived from one cell and are genetically identical

B. a gene from one organism has been inserted into a vector and successfully introduced into a host cell

C. all of the cells in a particular organism are identical

D. All of the above

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Question 39 : What is the name of the DNA molecule to which the gene of interest is integrated for cloning?

A. Vector

B. Template

C. Carrier

D. Transformer

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Question 40 : What are restriction endonucleases?

A. enzymes that process pre-RNA’s

B. enzymes that degrade DNA

C. enzymes that protect bacterial cells from viral infection

D. None of these

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Question 41 : The Human Genome Project (HGP) is closely tied to the rapid development of a new area of biology called?

A. Biotechnology

B. Bioinformatics

C. Biogeography

D. Bioscience

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Question 42 : Which of the following statements about restriction enzymes is incorrect?

A. They work on DNA extracted from all types of organisms

B. They are used to glue together short segments of DNA

C. They come in many varieties, each with its DNA target sequence

D. They are highly specific for their DNA target sequences

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Question 43 : Why do colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria?

A. Insertional inactivate of alpha-galactosidase in nonrecombinant bacteria

B. Insertional inactivation of alpha-galactosidase in recombinant bacteria

C. Inactivation of glycosidase enzyme in recombinant bacteria

D. Non-recombinant bacteria containing beta-galactosidase

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Question 44 : In a PCR reaction, which of the following steps is catalyzed by Taq polymerase?

A. Denaturation of template DNA

B. Annealing of primers to template DNA

C. Extension of primer end on the template DNA

D. All of the above

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Question 45 : What is the use of electrophoresis?

A. separate fragments of DNA.

B. clone genes.

C. cut DNA into fragments.

D. match a gene with its function.

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Question 46 : In experiments involving DNA hybridization, which of the following is a critically important tool?

A. DNA sequencing machines

B. Ligases

C. DNA probes

D. Vectors

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Question 47 : What technique is used for separating DNA fragments?

A. Gel electrophoresis

B. Chromatography

C. Transformation

D. Transduction

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Question 48 : For a prokaryotic vector to be propagated in a bacterial host cell, the vector needs?

A. telomeres

B. centromeres

C. drug-resistant genes

D. an origin of replication

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Question 49 : The criterion for DNA fragment movement on agarose gel during gel electrophoresis is?

A. The smaller the fragment size, the farther it moves

B. Positively charged fragments move to the farther end

C. Negatively charged fragments do not move

D. The larger the fragment size, the farther it moves

Show Answer

Question 50 : What is a gene whose expression helps to identify a transformed cell?

A. Vector

B. Plasmid

C. Structural gene

D. Selectable marker

Show Answer

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